14-3-3 gamma anticorps (AA 107-138)

N° du produit ABIN7599673

Cet anticorps Lapin Polyclonal détecte spécifiquement 14-3-3 gamma dans WB, ELISA et FACS. Il présente une réactivité envers Humain, Souris et Rat.

Aperçu rapide pour 14-3-3 gamma anticorps (AA 107-138) (ABIN7599673)

Antigène: 14-3-3 gamma (YWHAG1)

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp 14-3-3 gamma est non-conjugé

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Détail du produit anti-14-3-3 gamma anticorps

Épitope: AA 107-138

Fonction: Anti-14-3-3 gamma/YWHAG Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-14-3-3 gamma/YWHAG Antibody (ABIN7599673). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human 14-3-3 gamma/YWHAG recombinant protein (Position: Y107-A138).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Autieri, M. V., Carbone, C. J. 14-3-3-Gamma interacts with and is phosphorylated by multiple protein kinase C isoforms in PDGF-stimulated human vascular smooth muscle cells. DNA Cell Biol. 18: 555-564, 1999. 2. Cornell, B., Wachi, T., Zhukarev, V., Toyo-oka, K. Overexpression of the 14-3-3-gamma protein in embryonic mice results in neuronal migration delay in the developing cerebral cortex. Neurosci. Lett. 628: 40-46, 2016. 3. Horie, M., Suzuki, M., Takahashi, E., Tanigami, A. Cloning, expression, and chromosomal mapping of the human 14-3-3gamma gene (YWHAG) to 7q11.23. Genomics 60: 241-243, 1999.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur 14-3-3 gamma

Antigène: 14-3-3 gamma (YWHAG1)

Autre désignation: YWHAG

Sujet:

Synonyms: Prostaglandin E synthase 3, Cytosolic prostaglandin E2 synthase, Cpges, Hsp90 co-chaperone, Progesterone receptor complex p23, Telomerase-binding protein p23, PTGES3, P23, TEBP

Tissue Specificity: Mainly expressed in testis. Isoform 3 is expressed predominantly in adult testis, weakly in pancreas, embryonic testis and sperm, and at very low levels in other organs.

Background: This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100 % identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways.

Poids moléculaire: 28 kDa

ID gène: 7532

UniProt: P61981

Pathways: Myometrial Relaxation and Contraction, M Phase